Thermo Fisher Scientific PIP5K1A Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500

-

Flow Cytometry (Flow)

1:500-1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein within human PIP5K1A aa 400-562 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PIP5K1A,uniProtId:Q99755-1,ncbiNodeId:9606,antigenRange:400-562,antigenLength:562,antigenImageFileName:PA5-119860_PIP5K1A_Q99755-1_Rabbit.svg,antigenImageFileNamePDP:PA5-119860_PIP5K1A_Q99755-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7.4, with 50% glycerol, 0.2% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2913432

Product Specific Information

Positive Control: 293T cell lysate, SiHa cell lysate, SK-Br-3 cell lysate, rat skeletal muscle tissue, human tonsil tissue, human liver tissue, mouse testis tissue, SiHa.

Subcellular Location: Cell membrane, Nucleus, Nucleus speckle, Cytoplasm, ruffle, lamellipodium.

Target Information

PIP5K1A lipid kinase or phosphatidylinositol-4-phosphate 5-kinase 1 alpha phosphorylates phosphatidylinositol-4-phosphate to form phosphatidylinositol 4,5-bisphosphate. PIP5K1 is required for Wnt3a-induced LRP6 phosphorylation at ser1490 in mammalian cells. Wnt3a stimulates the formation of phosphatidylinositol 4,5-bisphosphates through frizzled and disheveled, the latter of which directly interacts with and activated PIP5KI. PIP5K1A-mediated PIP(2) production is crucial for HIV-1 entry and the early steps of infection in permissive lymphocytes. Overexpression of wild-type PI4P5K1A increases HIV-1 Env-mediated PIP(2) production and enhances viral replication in primary lymphocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.