Thermo Fisher Scientific Collagen IV Polyclonal Antibody
Rabbit polyclonal antibody targeting human Collagen IV. Validated for WB and ICC/IF. Provides strong specificity for basement membrane protein detection. Supplied as liquid, 1 mg/mL, antigen affinity purified. For research use only.
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- PA5119856
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein within C-terminal human Collagen IV |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.4, with 50% glycerol, 0.2% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4 °C short term; for long term, store at −20 °C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2913428 |
Product Specific Information
- Positive Control: MCF‑7 cell lysate, SH‑SY5Y cell lysate, SH‑SY5Y
- Subcellular Location: Basement membrane
Target Information
Collagen IV is a major structural component of the basement membrane found in tissues such as kidney, lung, and skin. It forms a network-like scaffold providing mechanical support and stability. Collagen IV consists of three alpha chains encoded by COL4A1, COL4A2, and COL4A3 genes. Mutations in these genes are associated with disorders such as Alport syndrome and Goodpasture syndrome, which primarily affect the kidneys and lungs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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