Thermo Fisher Scientific ER-beta (Estrogen Receptor beta-2) Monoclonal Antibody (57/3)
ER-beta2 이소폼을 인식하는 Thermo Fisher Scientific의 단클론 항체로, Western blot, IHC, ICC, Flow cytometry 등에 사용 가능. 사람 시료 반응성, Mouse IgG1, Protein A/G 정제, PBS buffer, 보존제 없음, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 2–4 µg/mL |
| Flow Cytometry (Flow) | 2–4 µg/10⁶ cells |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 57/3 |
| Immunogen | A synthetic peptide (CGMKMETLLPEATMEQ) from the C-terminus of the human estrogen receptor beta 2 isoform |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
Target Information
This monoclonal antibody recognizes the estrogen receptor beta 2 isoform, expressed in the nuclei of various human tissues including ovary, placenta, testis, and vas deferens. It shows no cross-reactivity with the estrogen receptor beta 1 isoform.
The encoded gene belongs to the estrogen receptor and nuclear receptor transcription factor superfamily. The protein contains an N-terminal DNA-binding domain and a C-terminal ligand-binding domain, and is localized to the nucleus, cytoplasm, and mitochondria.
Upon binding to 17β-estradiol or related ligands, it forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms can dominantly inhibit other estrogen receptor family members.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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