Thermo Fisher Scientific IL-4 Monoclonal Antibody (3010)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

ELISA (ELISA)

0.1-0.4 µg/mL (Detection), 2-8 µg/mL (Capture)

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3010

Immunogen

E. coli-derived recombinant human IL-4 His25-Ser153 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:IL-4,uniProtId:P05112-1,ncbiNodeId:9606,antigenRange:25-153,antigenLength:153,antigenImageFileName:MA5-23692_IL-4_P05112-1_House_mouse.svg,antigenImageFileNamePDP:MA5-23692_IL-4_P05112-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2609714

Product Specific Information

In sandwich ELISAs, no cross-reactivity or interference was observed with recombinant human (rh) IL-1 alpha, rhIL-1 beta, rhIL-2, rhIL-3, rhIL-6, rhIL-7, rhIL-8, rhG-CSF, rhGM-CSF, rhTNF-alpha, rhTNF- beta, rhLIF, rhTGF- beta 1, recombinant mouse (rm) IL-1 beta, rmIL-6, rmIL-7, rmGM-CSF, bovine FGF acidic, bovine FGF basic, porcine PDGF, human TGF- beta 1, porcine TGF- beta 1.2, and porcine TGF- beta 2.

Reconstitute at 0.5 mg/mL in sterile PBS.

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.