Thermo Fisher Scientific CD99 Monoclonal Antibody (SP119)
Rabbit monoclonal antibody (clone SP119) targeting human CD99 for IHC (paraffin) applications. Provides specific detection of CD99 involved in T-cell regulation. Protein A/G purified, liquid form, stored at 4°C or -20°C. For research use only.
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Applications
- Immunohistochemistry (Paraffin) (IHC (P))
Tested Dilution: 1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SP119 |
| Immunogen | Recombinant full length human CD99 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.6, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2537935 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature.
A suggested positive control is pancreas tissue.
Target Information
CD99 (E2, MIC2) is a transmembrane glycoprotein involved in regulation of T cell adhesive properties and programmed cell death distinct from typical apoptosis.
Its roles are specific to certain stages of T cell differentiation such as corticothymocytes.
CD99R isoform expression is restricted in the hematopoietic system to T, NK, and myeloid cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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