Thermo Fisher Scientific Vimentin Monoclonal Antibody (SP20)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 10 publications 10 publications

Immunohistochemistry (IHC)

-

View 21 publications 21 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:1,000

View 9 publications 9 publications

Flow Cytometry (Flow)

1:100

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Non-human primate, Sheep

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

SP20

Immunogen

Recombinant human vimentin protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.006 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.20, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2537928

Product Specific Information

Predicted to react with Rat, Hamster, Cow, and Xenopus laevis based on sequence homology. This antibody is not suitable for mouse tissues.

Recommended Postive Controls - WB: U-2 OS, Hu tonsil and HeLa cell lysates. Flow: HeLa cells. ICC/IF: HAP1-VIM cells, human limbal epithelial cells. IHC-P: Human breast cancer and melanoma tissue. IHC-Fr: Colorectal cancer tissue.

Target Information

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.