Thermo Fisher Scientific Vimentin Monoclonal Antibody (SP20)
Rabbit monoclonal antibody (clone SP20) targeting human vimentin. Validated for WB, IHC, ICC/IF, and Flow Cytometry. Recognizes mesenchymal-origin protein involved in cytoskeletal transport and steroid synthesis. Suitable for research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 10 publications |
| Immunohistochemistry (IHC) | - | View 21 publications |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200 | View 2 publications |
| Immunocytochemistry (ICC/IF) | 1:1,000 | View 9 publications |
| Flow Cytometry (Flow) | 1:100 | View 1 publication |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse, Non-human primate, Sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SP20 |
| Immunogen | Recombinant human vimentin protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.006 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.20, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2537928 |
Product Specific Information
Predicted to react with Rat, Hamster, Cow, and Xenopus laevis based on sequence homology.
This antibody is not suitable for mouse tissues.
Recommended Positive Controls:
- WB: U-2 OS, human tonsil, and HeLa cell lysates
- Flow: HeLa cells
- ICC/IF: HAP1-VIM cells, human limbal epithelial cells
- IHC-P: Human breast cancer and melanoma tissue
- IHC-Fr: Colorectal cancer tissue
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It plays a role in intracellular protein transport between the nucleus and plasma membrane.
Unlike other IFPs, vimentin is co-expressed with desmin during early cellular development, later replaced by tissue-specific IFPs.
Vimentin contributes to steroid synthesis regulation by serving as a storage network for cholesterol-containing lipid droplets.
Phosphorylation by protein kinase leads to filament breakdown and activation of contractile events, facilitating lipid droplet interaction with mitochondria and enhancing steroid synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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