Thermo Fisher Scientific Cul5 Polyclonal Antibody
Cul5 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, IP 등 다양한 응용에 적합합니다. Human과 Mouse에 반응하며 Antigen affinity chromatography로 정제되었습니다. 액상 형태로 4°C에서 보관하며 연구용으로 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 5–15 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1 and 50 of human cullin-5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7–8, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
- Based on 100% sequence identity, this antibody is predicted to react with Rat, Rabbit, and Orangutan.
Target Information
CUL5 is a core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein, it may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAK2. It is also involved in proteasomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein and may form a cell surface vasopressin receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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