Thermo Fisher Scientific Cul5 Polyclonal Antibody
Rabbit polyclonal antibody targeting human Cullin-5 (residues 1–50). Suitable for WB, IHC, IHC-P, and IP applications. Unconjugated liquid form, 200 µg/mL concentration. Predicted reactivity with Rat, Rabbit, and Orangutan. Stable for 1 year at 4°C.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 5–15 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1 and 50 of human Cullin-5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Predicted to react with Rat, Rabbit, and Orangutan based on 100% sequence identity
Target Information
CUL5 is a core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein, CUL5 may contribute to catalysis by positioning both substrate and ubiquitin-conjugating enzyme. The functional specificity of the E3 ligase complex depends on the variable substrate recognition component. ECS(SOCS1) appears to direct ubiquitination of JAK2 and may be involved in proteasomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. It may also form a cell surface vasopressin receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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