Thermo Fisher Scientific WNT6 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

5-15 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Sheep / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived recombinant human Wnt-6 Met35-Glu75 and Gln238-Arg299 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:WNT6,uniProtId:Q9Y6F9-1,ncbiNodeId:9606,antigenRange:35-75|238-299,antigenLength:365,antigenImageFileName:PA5-47580_WNT6_Q9Y6F9-1_Sheep.svg,antigenImageFileNamePDP:PA5-47580_WNT6_Q9Y6F9-1_Sheep_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2576272

Product Specific Information

In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse (rm) Wnt-4, rmWnt-8a, and rmWnt-9b is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Target Information

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is overexpressed in cervical cancer cell line and strongly coexpressed with another family member, WNT10A, in colorectal cancer cell line. The gene overexpression may play key roles in carcinogenesis. This gene and the WNT10A gene are clustered in the chromosome 2q35 region. The protein encoded by this gene is 97% identical to the mouse Wnt6 protein at the amino acid level.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.