Thermo Fisher Scientific c-Met Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1 µg/mL

-

ELISA (ELISA)

0.1-0.4 µg/mL (Detection), 0.2-0.8 µg/mL (Capture)

-

Neutralization (Neu)

Assay-Dependent

-

Product Specifications

Species Reactivity

Dog

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant canine HGF R/c-MET Glu25-Leu935 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:c-Met,uniProtId:Q75ZY9-1,ncbiNodeId:9615,antigenRange:25-935,antigenLength:1382,antigenImageFileName:PA5-47585_c-Met_Q75ZY9-1_Goat.svg,antigenImageFileNamePDP:PA5-47585_c-Met_Q75ZY9-1_Goat_PDP.jpeg,sortOrder:4}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2607127

Product Specific Information

In sandwich ELISAs, less than 0.2% cross-reactivity with recombinant human HGF R and remombinant mouse HGF R is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

MET (cMET) is a receptor-like tyrosine kinase whose dysregulation has been linked to many types of human malignancies. After activation of the ligand, MET interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, and STAT3. There interactions lead to the activation of signaling cascades including RAS-ERK, PI3, kinase-AKT, and PLCgamma-PKC. MET plays a role in embryonic development including gastrulation, development of muscles and neurons, angiogenesis, and kidney formation. It also plays a role in adults including wound healing, organ regeneration, and tissue remodeling. MET has been linked to cancers including gastric, renal, and breast; therefore, making it a target for cancer therapeutics and diagnostic testing.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.