Thermo Fisher Scientific GTF2E1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:2,000

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 230-439 of human GTF2E1 (NP_0055042) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GTF2E1,uniProtId:P29083-1,ncbiNodeId:9606,antigenRange:230-439,antigenLength:439,antigenImageFileName:PA5-90339_GTF2E1_P29083-1_Rabbit.svg,antigenImageFileNamePDP:PA5-90339_GTF2E1_P29083-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.82 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2806033

Product Specific Information

Immunogen sequence: LAGGHHREAW ATKGPSYEDL YTQNVVINMD DQEDLHRASL EGKSAKERPI WLRESTVQGA YGSEDMKEGG IDMDAFQERE EGHAGPDDNE EVMRALLIHE KKTSSAMAGS VGAAAPVTAA NGSDSESETS ESDDDSPPRP AAVAVHKREE DEEEDDEFEE VADDPIVMVA GRPFSYSEVS QRPELVAQMT PEEKEAYIAM GQRMFEDLFE; Positive Samples: HeLa, Jurkat, A-431, Mouse brain, Mouse spleen, Mouse testis; Cellular Location: Nucleus

Target Information

The general transcription factor TFIIE has an essential role in eukaryotic transcription initiation together with RNA polymerase II and other general factors. Human TFIIE consists of two subunits of relative molecular mass 57, 000 (TFIIE-alpha) and 34, 000 (TFIIE-beta) and joins the preinitiation complex after RNA polymerase II and TFIIF. TFIIE-alpha is necessary for transcription initiation together with TFIIE-beta, and recombinant TFIIE-alpha can fully replace the natural subunit in an in vitro transcription assay. Its sequence contains several interesting structural motifs (leucine repeat, zinc finger and helix-turn-helix) and sequence similarities to bacterial sigma factors that suggest direct involvement in the regulation of transcription initiation.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.