Thermo Fisher Scientific CLEC2D Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1 µg/mL

-

Flow Cytometry (Flow)

2.5 µg per million cells

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived recombinant human OCIL/CLEC2d Ser57-Val191 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CLEC2D,uniProtId:Q9UHP7-1,ncbiNodeId:9606,antigenRange:57-191,antigenLength:191,antigenImageFileName:PA5-47496_CLEC2D_Q9UHP7-1_Goat.svg,antigenImageFileNamePDP:PA5-47496_CLEC2D_Q9UHP7-1_Goat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2576691

Product Specific Information

In Western blots, approximately 15% cross-reactivity with recombinant mouse (rm) OCIL is observed and less than 1% cross-reactivity with rmOCILrp2, recombinant human (rh) CLEC4D, and rhCLEC4F is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Target Information

Recently, Zhou et al have identified a protein that can inhibit multinucleate Osteoclast formation in murine osteoblast and spleen cell co-cultures. This protein called, Osteoclast Inhibitory Lectin (OCIL) can also inhibit osteoclast formation in spleen cell cultures treated with RANKL and M-CSF. Furthermore, mOCIL acted directly on macrophage/monocyte cells as evidenced by its inhibitory action on adherent spleen cell cultures, which were depleted of stromal and lymphocytic cells. mOCIL completely inhibited osteoclast formation during the proliferative phase of osteoclast formation and resulted in 70% inhibition during the differentiation phase. Osteoblast OCIL mRNA expression was enhanced by parathyroid hormone, calcitriol, interleukin-1 and IL-11, and retinoic acid. OCIL is expressed in osteoblasts and chondrocytes as well as in a variety of extraskeletal tissues. Since OCIL expression was found to be very similar to distribution for RANKL, it has been suggested that RANKl and OCIL may interact in skeleton and in extracellular tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.