Thermo Fisher Scientific Eotaxin 2 Polyclonal Antibody, Biotin, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

ELISA (ELISA)

0.25-1.0 µg/mL

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Goat

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Murine Eotaxin-2 (CCL24) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Eotaxin 2,uniProtId:Q9JKC0-1,ncbiNodeId:10090,antigenRange:27-119,antigenLength:119,antigenImageFileName:500-P175GBT-500UG_Eotaxin_2_Q9JKC0-1_Goat.svg,antigenImageFileNamePDP:500-P175GBT-500UG_Eotaxin_2_Q9JKC0-1_Goat_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

AA Sequence of recombinant protein: VTIPSSCCTS FISKKIPENR VVSYQLANGS ICPKAGVIFI TKKGHKICTD PKLLWVQRHI QKLDAKKNQP SKGAKAVRTK FAVQRRRGNS TEV.

Preparation: Produced from sera of goats immunized with highly pure Recombinant Murine Eotaxin-2 (CCL24). Anti-Murine Eotaxin-2 (CCL24)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect mEotaxin-2 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine Eotaxin-2 (500-P175G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mEotaxin-2.

Western Blot: To detect mEotaxin-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mEotaxin-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P175GBT-1MG will be provided as 2 x 500 µg

Target Information

Eotaxin 2 belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity on resting T lymphocytes, a minimal activity on neutrophils, and is negative on monocytes and activated T lymphocytes. The protein is also a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.