Thermo Fisher Scientific Eotaxin 2 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
VTIPSSCCTS FISKKIPENR VVSYQLANGS ICPKAGVIFI TKKGHKICTD PKLLWVQRHI QKLDAKKNQP SKGAKAVRTK FAVQRRRGNS TEV

Preparation:
Produced from sera of goats immunized with highly pure Recombinant Murine Eotaxin-2 (CCL24). Anti-Murine Eotaxin-2 (CCL24)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect mEotaxin-2 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine Eotaxin-2 (500-P175G) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant mEotaxin-2.

Western Blot:
To detect mEotaxin-2 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant mEotaxin-2 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Packaging:
500-P175GBT-1MG is provided as 2 × 500 µg.

Target Information

Eotaxin-2 belongs to the subfamily of small cytokine CC genes. Cytokines are secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity on resting T lymphocytes, minimal activity on neutrophils, and no activity on monocytes or activated T lymphocytes. It also acts as a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.