Thermo Fisher Scientific Phospho-EphA2/EphA3/EphA4 (Tyr588, Tyr596) Polyclonal Antibody
Phospho-EphA2/EphA3/EphA4 (Tyr588, Tyr596) 인산화 부위를 인식하는 Rabbit Polyclonal Antibody. Western blot 및 ICC/IF에 적합하며, HepG2 및 HeLa 세포를 양성 대조군으로 권장. 높은 특이성과 재현성을 제공하며, 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:1,000
- Suggested Positive Control: HepG2 cells
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100–1:200
- Suggested Positive Control: HeLa cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 588/596 (K-T-Y(p)-V-R)/(R-T-Y(p)-V-D) derived from Human EPHA2/3/4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2554419 |
Target Information
The Eph subfamily represents the largest group of receptor protein tyrosine kinases identified to date. Although their biological activities are not fully determined, evidence suggests involvement in central nervous system function and development.
Human Eph receptors and their homologs include:
- EphA1 (Eph)
- EphA2 (Eck)
- EphA3 (Hek4)
- EphA4 (Hek8)
- EphA5 (Hek7)
- EphA6 (Hek12)
- EphA7 (Hek11/MDK1)
- EphA8 (Hek3)
- EphB1 (Hek6)
- EphB2 (Hek5)
- EphB3 (Cek10, Hek2)
- EphB4 (Htk)
- EphB5 (Hek9)
- EphB6 (Mep)
Ligands for Eph receptors include:
- Ephrin-A4 (LERK-4) binds EphA3 and EphB1
- Ephrin-A2 (Elf-1) binds EphA4
- Ephrin-A3 (Ehk1-L) binds EphA5
- Ephrin-B2 (Htk-L) binds EphB4 (Htk)
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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