Thermo Fisher Scientific Phospho-AKT Pan (Tyr315, Tyr316, Tyr312) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

-

Immunocytochemistry (ICC/IF)

1:100-1:200

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide sequence around phosphorylation site of tyrosine 315/316/312 (P-E-Y(p)-L-A) derived from Human AKT1/AKT2/AKT3. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:AKT Pan,uniProtId:P31749-1,ncbiNodeId:9606,antigenRange:312-317|313-317,antigenLength:480,antigenImageFileName:PA5-37814_AKT_Pan_P31749-1_Rabbit.svg,antigenImageFileNamePDP:PA5-37814_AKT_Pan_P31749-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:AKT Pan,uniProtId:P31751-1,ncbiNodeId:9606,antigenRange:312-317|314-318,antigenLength:481,antigenImageFileName:PA5-37814_AKT_Pan_P31751-1_Rabbit.svg,antigenImageFileNamePDP:PA5-37814_AKT_Pan_P31751-1_Rabbit_PDP.jpeg,sortOrder:2},{targetFamily:AKT Pan,uniProtId:Q9Y243-1,ncbiNodeId:9606,antigenRange:310-314|312-317,antigenLength:479,antigenImageFileName:PA5-37814_AKT_Pan_Q9Y243-1_Rabbit.svg,antigenImageFileNamePDP:PA5-37814_AKT_Pan_Q9Y243-1_Rabbit_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2554422

Product Specific Information

A suggested positive control for Western blot is HepG2 cells; suggested positive control for IHC is human breast carcinoma; suggested positive control for ICC/IF is Hela cells.

Target Information

AKT also known as protein kinase B (PKB) or RAS-alpha, is an ubiquitous serine/threonine kinase that plays an important role in diverse biological responses such as regulation of metabolism, cell survival and growth by phosphorylating multiple proteins. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.