Thermo Fisher Scientific Phospho-Ephrin B1/B2/B3 (Tyr324/311/318) Polyclonal Antibody
Human Ephrin B1/B2/B3의 인산화 부위(Tyr324/311/318)를 인식하는 Rabbit Polyclonal Antibody. Western blot 및 IHC(Paraffin) 검증 완료. 액상 형태, 1 mg/mL, -20°C 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 324/311/318 (G-D-Y(p)-G-H) derived from Human Ephrin B1/B2/B3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2554418 |
Product Specific Information
- Suggested positive control for Western blot: K562 cells
- Suggested positive control for IHC: human brain tissue
Target Information
Eph receptors and ephrins exhibit complementary expression in many tissues during embryogenesis, indicating that bidirectional activation of Eph receptors and ephrin-B proteins may occur at expression domain interfaces.
Ephrin-B1 transduces outside-in signals through C-terminal protein interactions that affect integrin-mediated cell attachment and migration.
The distribution of ephrin-B1 in the developing retina suggests that it influences retinal axon mapping along the dorsal-ventral axis and may be involved in intratectal development.
The transmembrane ligand ephrin-B2 and its receptor tyrosine kinase EphB4 are specifically expressed on arterial and venous endothelial cells, respectively.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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