Thermo Fisher Scientific IL-3 Polyclonal Antibody, PeproTech

Thermo Fisher Scientific IL-3 Polyclonal Antibody, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
APMTQTTSLK TSWVNCSNMI DEIITHLKQP PLPLLDFNNL NGEDQDILME NNLRRPNLEA FNRAVKSLQN ASAIESILKN LLPCLPLATA APTRHPIHIK DGDWNEFRRK LTFYLKTLEN AQAQQTTLSL AIF

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IL-3. Anti-Human IL-3-specific antibody was purified by affinity chromatography employing an immobilized Human IL-3 matrix.

Sandwich ELISA:
To detect Human IL-3 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-3 (500-P23Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human IL-3.

Western Blot:
To detect Human IL-3 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IL-3 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

Interleukin 3 (IL-3), also called Mast Cell Growth Factor (MCGF), Multi-Colony Stimulating Factor (multi-CSF), or Eosinophil-CSF (E-CSF), is produced by activated T cells, mast cells, and eosinophils. IL-3 is a hematopoietic growth factor that stimulates colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell, and monocytic lineages.
Most of these functions are enhanced or dependent on co-stimulation with other cytokines. Human and mouse IL-3 share only 29% sequence identity at the amino acid level, and the bioactivity of IL-3 is highly species specific — human IL-3 has no activity on mouse cells.
IL-3 is a potent growth-promoting cytokine capable of supporting proliferation of a broad range of hematopoietic cell types, and is involved in cell growth, differentiation, and apoptosis. It also possesses neurotrophic activity and may be associated with neurologic disorders.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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