Thermo Fisher Scientific IGFBP3 Polyclonal Antibody, Biotin, PeproTech
IGFBP3 단백질을 표적으로 하는 Biotin 결합 폴리클로날 항체로, Western blot 및 Sandwich ELISA에 최적화되어 있습니다. 고순도 재조합 인간 IGFBP3로 면역된 토끼 혈청에서 생산되었으며, 항원 친화 크로마토그래피로 정제 후 Biotin화되었습니다. 연구용으로만 사용 가능합니다.
- 카탈로그번호
- 500-P230BT-500UG
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IGFBP3 Polyclonal Antibody, Biotin, PeproTech
Thermo Fisher Scientific IGFBP3 Polyclonal Antibody, Biotin, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.25–1.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human IGF-BP3 |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
Product Specific Information
AA Sequence of recombinant protein:
GASSGGLGPV VRCEPCDARA LAQCAPPPAV CAELVREPGC GCCLTCALSE GQPCGIYTER CGSGLRCQPS PDEARPLQAL LDGRGLCVNA SAVSRLRAYL LPAPPAPGNA SESEEDRSAG EVESPSVSST HRVSDPKFHP LHSKIIIIKK GHAKDSQRYK VDYESQSTDT QNFSSESKRE TEYGPCRREM EDTLNHLKFL NVLSPRGVHI PNCDKKGFYK KKQCRPSKGR KRGFCWCVDK YGQPLPGYTT KGKEDVHCYS MQSK.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IGF-BP3. Anti-Human IGF-BP3-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
To detect hIGF-BP3 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IGFBP3 (500-P230) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of recombinant hIGFBP3.
Western Blot:
To detect hIGFBP3 by Western blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for recombinant hIGFBP3 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Package Information:
500-P230BT-1MG will be provided as 2 × 500 µg.
Target Information
IGFBP3 belongs to the insulin-like growth factor binding protein (IGFBP) family. It encodes a protein containing both IGFBP and thyroglobulin type-I domains. IGFBP3 forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either IGF-I or IGF-II, circulating in plasma to prolong IGF half-life and modulate receptor interactions.
Diseases associated with IGFBP3 include Insulin-Like Growth Factor I and Acid-Labile Subunit Deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일은 본문 하단에 위치합니다.)
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.