Thermo Fisher Scientific NEFM Monoclonal Antibody (3H11)
인간 및 랫트 신경섬유 중간사슬(NF-M)을 검출하는 Thermo Fisher Scientific의 단클론 항체입니다. Western blot, IHC, ICC/IF, IP 등 다양한 응용에 적합하며, 1 mg/mL 농도의 액상 형태로 제공됩니다. -20°C에서 보관하며 Protein G로 정제되었습니다.
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific NEFM Monoclonal Antibody (3H11)
Thermo Fisher Scientific NEFM Monoclonal Antibody (3H11)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:100–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:500 |
| Immunohistochemistry (PFA fixed) (IHC-PFA) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC-F) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Published Species | Human |
| Host / Isotype | Mouse |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3H11 |
| Immunogen | Synthetic peptide corresponding to a C-terminal fragment of rat neurofilament |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_347004 |
Product Specific Information
MA1-2011 detects the neurofilament, medium chain (NF-M) in human and rat samples.
It has been successfully used in Western blot, immunofluorescence, and immunoprecipitation procedures.
By Western blot, this antibody detects a ~160 kDa protein representing the neurofilament medium chain in HEK 293 cells, rat spinal cord, and human frontal cortex.
In immunofluorescence procedures, MA1-2011 recognizes the neurofilament medium chain in human neurons.
The immunogen is a synthetic peptide corresponding to a C-terminal fragment of rat neurofilament.
Target Information
Neurofilaments (NFs) are intermediate filaments expressed almost exclusively in neuronal cells, especially in large axons.
They are composed of three polypeptide chains—NF-M (medium), NF-H (high), and NF-L (light)—which share structural similarity but differ in terminal sequences.
NF-M tail-mediated interactions are critical for axonal size and cytoskeletal architecture.
NF-M phosphorylation and O-GlcNAcylation regulate its translocation and filament formation.
Antibodies to neurofilament subunits are useful neuronal markers due to their abundance and stability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 없음)
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.