Thermo Fisher Scientific MTCO1 Polyclonal Antibody

Thermo Fisher Scientific MTCO1 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody was affinity-purified from rabbit antiserum by affinity chromatography using an epitope-specific immunogen. The purity is >95% (by SDS-PAGE).

Target Information

Cytochrome c oxidase subunit I (CO1 or MTCO1) is one of three mitochondrial DNA (mtDNA) encoded subunits (MTCO1, MTCO2, MTCO3) of respiratory Complex IV.
Complex IV, located within the mitochondrial inner membrane, is the third and final enzyme of the electron transport chain in mitochondrial oxidative phosphorylation. It consists of 13 polypeptides:

• Subunits I, II, and III (MTCO1, MTCO2, MTCO3) are mtDNA-encoded
• Subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc, and VIII are nuclear-encoded

The cytochrome c oxidase family of enzymes contains four redox centers (two hemes and two copper centers). In mitochondrial Complex IV, the two hemes are a and a3, and the two coppers are CuA and CuB. The hemes and CuB are bound to subunit I.
Research by Acin-Perez et al. (2003) identified cell lines with single and double missense mutations in the cytochrome c oxidase (COX) subunit I gene of mouse mitochondrial DNA. They hypothesized that deleterious mutations can arise and become predominant; cultured cells can maintain several mtDNA haplotypes at stable frequencies; the respiratory chain has little spare COX capacity; and that the cavity size near val421 in MTCO1I may affect enzyme function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.