Thermo Fisher Scientific Caspase 1 Monoclonal Antibody (14F468)
Caspase-1 단백질을 인식하는 mouse monoclonal antibody (clone 14F468). Western blot, IHC 등 다양한 응용에 적합하며 Human, Mouse, Rat 반응성 확인됨. Protein G 정제, PBS buffer, 1 mg/mL 농도로 제공.
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Applications 및 Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5–2 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | – | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:10–1:500 | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | – |
| Immunocytochemistry (ICC/IF) | Assay-dependent | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 14F468 |
| Immunogen | Two synthetic peptides from the human Caspase-1 protein (amino acids 371–390 and 31–45) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2537733 |
Product Specific Information
Suggested positive control: 3T3 whole cell lysate, antigen standard for CASP1 (transient overexpression lysate), HeLa or NIH-3T3, HeLa whole cell lysate.
Target Information
Caspases are a family of cysteine proteases divided into apoptotic and inflammatory subfamilies. Unlike apoptotic caspases, inflammatory caspases (such as caspase-1, -4, -5, and -12) are associated with immune responses to microbial pathogens and can activate proinflammatory cytokines such as IL-1β and IL-18. Caspase-1 was initially identified as an IL-1β-converting enzyme and later found to be a mammalian homolog of the C. elegans cell death gene ced-3, whose overexpression can induce apoptosis in fibroblasts.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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