
Thermo Fisher Scientific Goat anti-Human IgM (Heavy chain) Secondary Antibody, HRP
HRP 결합 염소 항-인간 IgM(Heavy chain) 2차 항체로, Western blot, IHC, ELISA에 적합합니다. 친화 크로마토그래피로 정제되었으며 높은 특이성과 재현성을 보장합니다. 동결건조 형태로 제공되며, 재구성 후 장기 보관 시 -20°C에서 안정합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Human Mu immunoglobulin |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2535612 |
| Target | IgM (Heavy chain) |
| Antibody Form | Whole Antibody |
Additional Formats Available
- AP conjugate
- Biotin conjugate
- FITC conjugate
- TRITC conjugate
- Custom conjugation available upon request
Product Specific Information
Each lot of antibody is tested for sensitivity using ELISA and for specificity by immunoelectrophoresis (IEP).
Reconstitution and Storage:
- Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature.
- Centrifuge to remove particulates.
- Prepare fresh working dilution daily.
- Store lyophilized material at 2–8°C.
- For long-term storage after reconstitution, dilute with 50% glycerol and store at -20°C as a liquid.
Specificity:
No reactivity observed to non-immunoglobulin human serum proteins or light chains on all human immunoglobulins.
Target Information
Anti-Human secondary antibodies are affinity-purified with well-characterized specificity for human immunoglobulins. They are useful for detection, sorting, or purification of target proteins.
Secondary antibodies provide versatility across detection systems (HRP, AP, fluorescence) and enhance sensitivity through signal amplification.
Typically generated by immunizing host animals with pooled human immunoglobulins, then purified and modified (immunoaffinity chromatography, fragmentation, or conjugation) to yield highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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