Thermo Fisher Scientific alpha Actinin 1 Polyclonal Antibody
Human alpha Actinin 1을 인식하는 Rabbit Polyclonal Antibody로 다양한 응용( WB, IHC, ICC, Flow)에 적합. Recombinant protein(aa 388–619)을 면역원으로 사용. 고순도 Affinity chromatography 정제, 액상 형태, 안정적 PBS/glycerol buffer 구성.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| Flow Cytometry (Flow) | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein within Human alpha Actinin aa 388–619 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.2% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2854717 |
Product Specific Information
Positive Control: A431, rat colon tissue lysates, mouse colon tissue lysates, MG-63, SiHa, human colon cancer tissue, human kidney tissue, mouse colon tissue.
Target Information
Alpha Actinin 1 belongs to the spectrin gene superfamily, a diverse group of cytoskeletal proteins including alpha/beta spectrins and dystrophins. It is an actin-binding protein with multiple roles across cell types.
- In nonmuscle cells: found along microfilament bundles and adherens-type junctions, binding actin to the membrane.
- In muscle cells: localized to the Z-disc and dense bodies, anchoring myofibrillar actin filaments.
This gene encodes a nonmuscle, cytoskeletal alpha actinin isoform and maps to the same site as the erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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