Norgen MidRanger 1 kb DNA Ladder

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1kb 이상의 PCR 산물 및 클로닝 응용에 적합한 정량형 DNA 래더. 300bp~5000bp 사이 10개 밴드 구성. 사용이 간편한 premixed 포맷으로 높은 안정성과 재현성 제공. 실온 안정, 장기 보관 시 -20°C 권장.

브랜드: Norgen
카테고리: Other Organics
판매단위: pk

#Norgen #DNA Ladders Reagents #Reagents #R

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Norgen · Norgen MidRanger 1 kb DNA Ladder

Norgen MidRanger 1 kb DNA Ladder

SKU: 11700

Ideal for sizing larger PCR products (>1kb) and most cloning applications.

Features and Benefits

Ready-to-use
Quantitative
Highly stable
Precise
Ten discrete fragments ranging from 300 bp to 5000 bp

The Norgen MidRanger 1 kb DNA Ladder is prepared to ensure quality and batch-to-batch consistency. It contains ten discrete fragments ranging from 300 bp to 5000 bp and is ideal for sizing larger PCR products (>1kb) and most cloning applications.

Contents:
1 mL of premixed DNA ladder (0.5 µg/10 µL) in loading buffer (10 mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

Ladder Properties

Fragment	Size (bp)	Mass (ng)
1	5000	88
2	4000	78
3	3000	67
4	2500	58
5	2000	50
6	1500	43
7	1000	25
8	700	34
9	500	33
10	300	24

Extracted Data from Image:
bp / ng per 10 µL
5000 bp – 88 ng
4000 bp – 78 ng
3000 bp – 67 ng
2500 bp – 58 ng
2000 bp – 50 ng
1500 bp – 43 ng
1000 bp – 25 ng
700 bp – 34 ng
500 bp – 33 ng
300 bp – 24 ng
(10 µL on 1% agarose)

Recommended Use

Mix thoroughly. For best results, load 10 µL of DNA ladder per well.
For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5 µg/mL ethidium bromide for 30–40 minutes.
The table above shows the size and mass for each band based on 10 µL ladder per well.

Storage

Stable at room temperature. For longer-term storage, -20 °C is recommended.
This ladder was standardized using 10 µL of DNA per lane on a 0.8 cm thick, 13 × 15 cm, 1.0% agarose gel run in TAE buffer.

Documentation

Citations

Title	Detection and Genotyping of Helicobacter pylori among Gastric Ulcer and Cancer Patients from Saudi Arabia
Citation	Pakistan Journal of Medical Sciences, 2017
Authors	Bibi, F., Alvi, S. A., Sawan, S. A., Yasir, M., Sawan, A., Jiman-Fatani, A. A., & Azhar, E. I.

| Title | Genetic variation and its reflection on post translational modifications in frequency clock and mating type a-1 proteins in Sordaria fimicola |
| Citation | BioMed Research International, 2017 |
| Authors | Arif, R., Akram, F., Jamil, T., Mukhtar, H., & Saleem, M. |