Norgen MidRanger 1 kb DNA Ladder

Norgen MidRanger 1 kb DNA Ladder

SKU: 11700

Ideal for sizing larger PCR products (>1kb) and most cloning applications.

Features and Benefits

• Ready-to-use
• Quantitative
• Highly stable
• Precise
• Ten discrete fragments ranging from 300 bp to 5000 bp

The Norgen MidRanger 1 kb DNA Ladder is prepared to ensure quality and batch-to-batch consistency. It contains ten discrete fragments ranging from 300 bp to 5000 bp and is ideal for sizing larger PCR products (>1kb) and most cloning applications.

Contents:
1 mL of premixed DNA ladder (0.5 µg/10 µL) in loading buffer (10 mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

Ladder Properties

Extracted Data from Image:
bp / ng per 10 µL
5000 bp – 88 ng
4000 bp – 78 ng
3000 bp – 67 ng
2500 bp – 58 ng
2000 bp – 50 ng
1500 bp – 43 ng
1000 bp – 25 ng
700 bp – 34 ng
500 bp – 33 ng
300 bp – 24 ng
(10 µL on 1% agarose)

Recommended Use

Mix thoroughly. For best results, load 10 µL of DNA ladder per well.
For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5 µg/mL ethidium bromide for 30–40 minutes.
The table above shows the size and mass for each band based on 10 µL ladder per well.

Storage

Stable at room temperature. For longer-term storage, -20 °C is recommended.
This ladder was standardized using 10 µL of DNA per lane on a 0.8 cm thick, 13 × 15 cm, 1.0% agarose gel run in TAE buffer.

Documentation

• Product Insert (11700)
• Application Note: DNA Quantification by Gel Densitometry
• Safety Data Sheet (11700)

Citations

| Title | Genetic variation and its reflection on post translational modifications in frequency clock and mating type a-1 proteins in Sordaria fimicola |
| Citation | BioMed Research International, 2017 |
| Authors | Arif, R., Akram, F., Jamil, T., Mukhtar, H., & Saleem, M. |

| Title | Detection of DNA methylation pattern in thidiazuron-induced blueberry callus using methylation-sensitive amplification polymorphism |
| Citation | Biologia Plantarum, 2016 |
| Authors | Ghosh, A., Igamberdiev, A. U., & Debnath, S. C |

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