Thermo Fisher Scientific Phospho-S6 (Ser244, Ser247) Polyclonal Antibody
RPS6 단백질의 Ser244 및 Ser247 인산화 부위를 인식하는 Rabbit Polyclonal Antibody. Western blot 등 다양한 응용에 적합하며, 항원 친화 크로마토그래피로 정제됨. 인간, 마우스, 어류 등에서 반응. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | - |
| Immunohistochemistry (IHC) | - | 10 |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 2 |
| Immunohistochemistry - Free Floating (IHC (Free)) | - | 1 |
| Immunocytochemistry (ICC/IF) | - | 1 |
| Immunoprecipitation (IP) | - | 5 |
| In Situ Hybridization (ISH) | - | 1 |
| Miscellaneous PubMed (Misc) | - | 2 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Fish, Human, Mouse, Xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Chemically synthesized phosphopeptide derived from human RPS6 containing Ser244 and Ser247. Sequence conserved in mouse and rat. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Dulbecco’s PBS, pH 7.3, with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533798 |
Target Information
Ribosomes are organelles that catalyze protein synthesis, composed of small 40S and large 60S subunits containing 4 RNA species and ~80 distinct proteins. The encoded cytoplasmic ribosomal protein is part of the 40S subunit and belongs to the S6E family. It is a major substrate of protein kinases in the ribosome, with phosphorylation at five C-terminal serine residues by various kinases. Phosphorylation occurs in response to growth factors, tumor-promoting agents, and mitogens, while dephosphorylation is associated with growth arrest. This protein may regulate cell growth and proliferation via selective mRNA translation. Multiple processed pseudogenes exist throughout the genome.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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