Thermo Fisher Scientific GPI Monoclonal Antibody (OTI5A11), TrueMAB
GPI 단백질을 표적하는 Mouse IgG2b 단클론 항체로, Western blot, IHC, ICC/IF, Flow Cytometry에 적합합니다. Human 및 Dog 반응성이 있으며, Lyophilized 형태로 제공됩니다. 연구용으로 에너지 대사 관련 GPI 단백질 분석에 활용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5A11 |
| Immunogen | Full length human recombinant protein of human GPI produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The GPI gene encodes a multifunctional phosphoglucose isomerase involved in energy metabolism. The dimeric enzyme catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate.
Inside the cell, it functions in glycolysis and gluconeogenesis; outside the cell, it acts as a neurotrophic factor for spinal and sensory neurons.
Defects in this gene can cause nonspherocytic hemolytic anemia and may be associated with severe enzyme deficiency leading to hydrops fetalis, neonatal death, and neurological impairment.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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