Thermo Fisher Scientific TCP1 Monoclonal Antibody (4E215)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (IHC)

Assay-dependent

-

Immunocytochemistry (ICC/IF)

5 µg/mL

-

Immunoprecipitation (IP)

5 µg/mL

-

Product Specifications

Species Reactivity

Bovine, Dog, Hamster, Mouse, Sheep, Rabbit, Rat

Host/Isotype

Rat / IgG2c

Class

Monoclonal

Type

Antibody

Clone

4E215

Immunogen

Rats were immunized with a purified recombinant mouse TCP1 alpha construct encoding the C-terminal half of the 1.8 kb full-length Tcp-1b gene expressed in E. coli. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TCP1,uniProtId:P11983-1,ncbiNodeId:10090,antigenRange:1-556,antigenLength:556,antigenImageFileName:MA1-91539_TCP1_P11983-1_Rat.svg,antigenImageFileNamePDP:MA1-91539_TCP1_P11983-1_Rat_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.09% Sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2202381

Product Specific Information

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The T Complex Polypeptide 1 (TCP-1) is approximately 60 kDa protein constitutively expressed in almost all eukaryotic cells, and is upregulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60, Escherichia coli GroEL, the plastid Rubisco-subunit binding protein, and the archaebacterial protein TF55. The TCP-1 sequence shows nearly 40% identity to TF55, but only minimal similarity to HSP60 and GroEL.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.