Thermo Fisher Scientific TCP1 Monoclonal Antibody (4E215)
TCP1 단백질을 인식하는 Rat IgG2c 형 단클론 항체로, WB, IHC, ICC, IP 등 다양한 응용에 적합합니다. Bovine, Mouse, Rat 등 여러 종에 반응하며, Protein G로 정제된 액상 형태입니다. 연구용으로만 사용됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
| Immunoprecipitation (IP) | 5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Dog, Hamster, Mouse, Sheep, Rabbit, Rat |
| Host / Isotype | Rat / IgG2c |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4E215 |
| Immunogen | Rats were immunized with a purified recombinant mouse TCP1 alpha construct encoding the C-terminal half of the 1.8 kb full-length Tcp-1b gene expressed in E. coli. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.09% Sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2202381 |
Product Specific Information
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The T Complex Polypeptide 1 (TCP-1) is an approximately 60 kDa protein constitutively expressed in almost all eukaryotic cells and is upregulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone involved in the folding of actin and tubulin.
Chaperonins recognize and stabilize polypeptide intermediates during folding, assembly, and disassembly, sharing characteristics with Heat Shock Protein 70 (HSP70) such as high abundance, induction by environmental stress, and ATPase activity.
The chaperonin family includes mitochondrial HSP60, E. coli GroEL, plastid Rubisco-subunit binding protein, and archaebacterial protein TF55. TCP-1 shows nearly 40% identity to TF55 but only minimal similarity to HSP60 and GroEL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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