Thermo Fisher Scientific DGKI Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:200

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100–1:500

Product Specifications

Product Specific Information

Antibody detects endogenous levels of total DGKI.

Target Information

Diacylglycerol (DAG) influences numerous cell signaling cascades by functioning as an intracellular activator of protein kinase C (PKC) and guanine nucleotide exchange factors. Intracellular DAG levels are regulated by diacylglycerol kinases (DGKs), which phosphorylate DAG to phosphatidic acid.

Human DGK isoforms:

• Type I DGKs: DGK-α (80 kDa), -β (90 kDa), -γ (90 kDa) — contain calcium-binding EF-hand motifs.
• Type II DGKs: DGK-δ (130 kDa), DGK-ι (130 kDa) — contain N-terminal pleckstrin homology (PH) domains.
• Type III DGKs: DGK-ε (64 kDa) — no identifiable regulatory domains.
• Type IV DGKs: DGK-Ω (104 kDa), DGK-ι (130 kDa) — possess C-terminal ankyrin repeats.
• Type V DGKs: DGK-θ (110 kDa) — contains 3 cysteine-rich domains and a PH domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.