Thermo Fisher Scientific AKAP8 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000-1:2,500

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human AKAP8. Recombinant protein control fragment (Product #RP-102489). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:AKAP8,uniProtId:O43823-1,ncbiNodeId:9606,antigenRange:553-690,antigenLength:692,antigenImageFileName:PA5-52103_AKAP8_O43823-1_Rabbit.svg,antigenImageFileNamePDP:PA5-52103_AKAP8_O43823-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2637816

Product Specific Information

Immunogen sequence: PEMEGDDNLG GEDKKETPEE VAADVLAEVI TAAVRAVDGE GAPAPESSGE PAEDEGPTDT AEAGSDPQAE QLLEEQVPCG TAHEKGVPKA RSEAAEAGNG AETMAAEAES AQTRVAPAPA AADAEVEQTD AESKDAVP

Highest antigen sequence identity to the following orthologs: Mouse - 53%, Rat - 58%.

Target Information

The type II cAMP-protein kinase (PKA) is a multifunctional kinase with a broad range of substrates. Specificity of PKA signaling is thought to be mediated by the compartmentalization of the kinase to specific sites within the cell. To maintain this specific localization, the R subunit (RII) of PKA interacts with specific RII-anchoring proteins. The family of RII-anchoring proteins has been designated A-kinase anchoring proteins (AKAP). AKAP 95, also known as AKAP 8, is a nuclear matrix protein predominantly expressed in liver, heart, pancreas, kidney and skeletal muscle. During mitosis, AKAP 95 is recruited to the chromosomes and plays an essential role in mitotic progression. Characteristic of its family, AKAP 95 participates in PKA signaling through an interaction with the RII regulatory subunit. In addition, AKAP 95 forms a complex with HA95 and HDAC3 and is required for the deacetylation of Histone H3 in mitosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.