Thermo Fisher Scientific MCM3 Polyclonal Antibody
MCM3 단백질을 인식하는 Rabbit IgG 폴리클로날 항체로, Western blot과 IHC(P) 실험에 적합합니다. 고순도 항원 친화 크로마토그래피로 정제되었으며, 인간, 마우스, 랫트에 반응합니다. 연구용으로만 사용 가능합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 0.04–0.4 µg/mL
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:500–1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human MCM3. Recombinant protein control fragment (Product #RP-102497). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2643812 |
Product Specific Information
Immunogen sequence:
KMVSAAFMKK YIHVAKIIKP VLTQESATYI AEEYSRLRSQ DSMSSDTART SPVTARTLET LIRLATAHAK ARMSKTVDLQ DAEEAVELVQ YAYFKKVLEK EKKRKKRSED ESETEDEEEK SQEDQEQKRK RRKTR
- Highest antigen sequence identity to orthologs:
- Mouse: 95%
- Rat: 93%
Target Information
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) involved in the initiation of eukaryotic genome replication.
The hexameric MCM complex is a key component of the pre-replication complex (pre_RC), playing a role in replication fork formation and recruitment of other DNA replication-related proteins.
MCM3 interacts directly with MCM5/CDC46 and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. This acetylation inhibits DNA replication initiation and cell cycle progression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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