Thermo Fisher Scientific MCM3 Polyclonal Antibody
MCM3 단백질을 인식하는 Rabbit Polyclonal Antibody로 인간, 마우스, 랫트에 반응합니다. Western blot, IHC, ICC/IF에 적합하며 고순도 항원 친화 크로마토그래피로 정제되었습니다. 장기 보관 시 -20°C에서 보관 권장.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human MCM3. Recombinant protein control fragment (Product #RP-102498). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.05 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2643813 |
Product Specific Information
Immunogen sequence:
TYAKQYEEFY VGLEGSFGSK HVSPRTLTSC FLSCVVCVEG IVTKCSLVRP KVVRSVHYCP ATKKTIERRY SDLTTLVAFP SSSVYPTKDE ENNPLETEYG LSVYKDHQTI TIQEMPEKAP AGQLPRSVDV ILDDDLVDK
Highest antigen sequence identity to the following orthologs:
- Mouse: 99%
- Rat: 99%
Target Information
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication-related proteins. This protein is a subunit of the protein complex that consists of MCM2–7. It interacts directly with MCM5/CDC46 and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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