Thermo Fisher Scientific MRPL4 Polyclonal Antibody
Human MRPL4 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제되었으며, PBS/glycerol buffer에 보관. 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 | – |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human MRPL4. Recombinant protein control fragment (Product # RP-100664) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2644148 |
Product Specific Information
Immunogen sequence:
MLQFVRAGAR AWLRPTGSQG LSSLAEEAAR ATENPEQVAS EGLPEPVLRK VELPVPTHRR
PVQAWVESLR GFEQERVGLA DLHPDVFATA PRLDILHQVA- Highest antigen sequence identity to the following orthologs:
- Mouse: 68%
- Rat: 71%
Target Information
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified alternatively spliced variants that encode different protein isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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