Merck Anti-Cas9

Anti-Cas9

from rabbit, purified by affinity chromatography

SpCas9, CRISPR-associated endonuclease Cas9

생물학적 소스

rabbit

Quality Level

100

항체 형태

affinity isolated antibody

antibody product type

primary antibodies

클론

polyclonal

정제법

affinity chromatography

species reactivity

Streptococcus pyogenes, bacteria

포장

antibody small pack of 25 μg

technique(s)

immunocytochemistry: suitable
western blot: suitable

NCBI 수납 번호

NP_269215.1

UniProt 수납 번호

Q99ZW2

배송 상태

ambient

CRISPR-associated endonuclease Cas9/Csn1 (UniProt: Q99ZW2; also known as SpCas9, SpyCas9) is encoded by the Cas9 (also known as Csn1) gene (Gene ID: 901176) in Streptococcus pyogenes. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. Cas9 has a bi-lobed architecture with a recognition lobe (residues 41-425) and a discontinuous nuclease lobe (NUC, residues 1-40 and 453-1053); the crRNA-target DNA lies in a channel between the 2 lobes. The NUC lobe has 2 endonuclease domains. The discontinuous RuvC-like domain in NUC cleaves the target DNA non-complementary to crRNA while the HNH nuclease domain cleaves the target DNA complementary to crRNA. Cas9 serves as a RNA-guided DNA endonuclease that cleaves double-stranded DNA targets with a protospacer adjacent motif (PAM) and complementarity to the guide RNA. The homologous region of the Staphylococcus aureus is not closely related in amino acid sequence in Streptococcus pyogenes, hence this antibody does not recognize that protein.