Merck Anti-Actin antibody, Mouse monoclonal

Anti-Actin antibody, Mouse monoclonal

clone AC-40, purified from hybridoma cell culture

Quality Level

200

생물학적 소스

mouse

결합

unconjugated

항체 형태

purified from hybridoma cell culture

antibody product type

primary antibodies

클론

AC-40, monoclonal

form

buffered aqueous solution

포장

antibody small pack of 25 μL

technique(s)

immunocytochemistry: 10-20 μg/mL using chicken or human fibroblasts by methanol/acetone fixation
immunohistochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.5 μg/mL using chicken or human fibroblasts

배송 상태

dry ice

저장 온도

−20°C

Actin is a highly conserved protein that is a major component of both the cytoskeletal and contractile structures in all cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. Actin can be found in two different forms of aggregation, the globular or the fibrillar state, and at least six distinct isoforms occur in vertebrates. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three α actins (skeletal, cardiac, smooth), one β actin (β-non-muscle) and two γ actins (γ smooth muscle and γ non-muscle). The human γ-actin gene is mapped to human chromosome 17.
The antibody recognizes an epitope located on the C-terminal end of actin conserved in all actin isoforms. It specifically labels actin in a wide variety of tissues and species. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. Zinc-formalin, B5, ethanol, methacarn, Brunnel′s or Bouin′s solutions may also be used as fixatives.
Monoclonal Anti-Actin (mouse IgG2a isotype) is derived from the AC-40 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. A synthetic actin C-terminal peptide, attached to Multiple Antigen Peptide (MAP) backbone was used as the immunogen. The isotype is determined using Mouse Monoclonal Antibody Isotyping Reagents, Catalog No. ISO2