Merck Anti-LC3B antibody produced in rabbit

Anti-LC3B antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

생물학적 소스

rabbit

Quality Level

200

결합

unconjugated

항체 형태

affinity isolated antibody

antibody product type

primary antibodies

클론

polyclonal

form

buffered aqueous solution

분자량

antigen 16-18 kDa

species reactivity

rat, human, mouse

향상된 검증

functional assay
Learn more about Antibody Enhanced Validation

농도

~1 mg/mL

technique(s)

indirect immunofluorescence: 5-10 μg/mL using human HeLa cells
western blot: 1-2 μg/mL using whole extracts of rat and mouse brain

UniProt 수납 번호

Q9GZQ8

application(s)

research pathology

배송 상태

dry ice

저장 온도

−20°C

Gene Information

human ... MAP1LC3B(81631)
mouse ... Map1lc3b(67443)
rat ... Map1lc3b(64862)

Microtubule-associated protein 1 light chain 3 (MAPLC3/LC3) was first identified in rat as a protein that co-purifies with microtubule-associated protein 1A and 1B from rat brain. LC3 exists in cells in two forms. One is cytoplasmic, LC3-I (18 kDa) and the other, LC3-II (16 kDa) is associated with the autophagosome membrane. LC3-I is modified to a membrane-bound form, LC3-II (a LC3-phospholipid conjugate), by mammalian autophagy related protein 7 (Atg7) and Atg3, which are E1- and E2-like enzymes, respectively. Three human orthologs of the rat LC3, named MAP1LC3A/LC3A, MAP1LC3B/LC3B, and MAP1LC3C/ LC3C, were identified. The human proteins exhibit two forms representing the cytosolic (type I) and the membrane associated (type II) forms. The three human isoforms show different expression patterns in human tissues.