Merck Anti-Actin antibody produced in rabbit

Anti-Actin antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

생물학적 소스

rabbit

Quality Level

200

결합

unconjugated

항체 형태

affinity isolated antibody

antibody product type

primary antibodies

클론

polyclonal

form

buffered aqueous solution

분자량

antigen 42 kDa

species reactivity

wide range, human, chicken, amoeba, slime mold, vertebrates

향상된 검증

independent
Learn more about Antibody Enhanced Validation

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25 using human or animal tissue sections
indirect immunofluorescence: 1:40 using chicken fibroblasts
western blot: 1:100 using chicken gizzard extract

UniProt 수납 번호

P68133

application(s)

research pathology

배송 상태

dry ice

저장 온도

−20°C

Gene Information

human ... ACTA1(58)
mouse ... Acta1(11459)
rat ... Acta1(29437)

Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in all cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. Actin can be found in two different forms of aggregation, the globular or the fibrillar state, and at least six distinct isoforms occur in vertebrates. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence.
The ACTA1 (actin, α 1) gene is mapped to human chromosome 1q42.13. The gene codes for actin 1, a thin filament component of skeletal muscle.

In immunoblotting, the product localizes actin in many species ranging from human skeletal muscle to amoeba. The product recognizes the 42 kDa actin band using immunoblotting techniques in human or animal tissue extracts.