Merck MTP Taq DNA Polymerase

MTP™ Taq DNA Polymerase

Taq DNA Polymerase, free of DNA contaminants

DNA-free Taq DNA polymerase

Quality Level

200

form

liquid

사용

sufficient for 1500 reactions
sufficient for 250 reactions

특징

Difficult Templates/Specialty Enzymes PCR
High Fidelity PCR
dNTPs included: no
hotstart: no

농도

5 unit/μL

technique(s)

PCR: suitable

색상

colorless

input

purified DNA

배송 상태

wet ice

저장 온도

−20°C

MTP™ Taq DNA Polymerase is a recombinant thermostable enzyme from Thermus aquaticus expressed in E. coli and purified using a proprietary process to minimize levels of contaminating DNA. The enzyme has both 5′→3′ DNA polymerase and exonuclease activities, is ∼95 kDa by SDS-PAGE, and has no detectable endonuclease or 3′→5′ exonuclease activities. Each lot of MTP Taq undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA.

Contaminating DNA present in most other polymerase preparations often preclude or obscure the accurate interpretation of results, especially when targeting conserved sequences, e.g., bacterial 16S rRNA region. Through Sigma′s proprietary DNA removal methods and strict quality control standards, we can ensure the absence of the most commonly found contaminant DNA. Each lot of MTP Taq is assayed using PCR and primers specific to the conserved region of bacterial 16S rRNA, the Taq expression vector, and the human β-actin gene.

While MTP Taq ensures a high-quality, low contaminant DNA polymerase for reliable PCR amplification, DNA contaminants can be introduced into PCR through a number of other reagents. To further minimize the risk of contaminant DNA during PCR, we include 10× MTP Taq Buffer with each tube of MTP Taq DNA Polymerase. Each lot of 10× MTP Taq Buffer undergoes the same strict quality control testing as MTP Taq DNA polymerase to ensure the absence of contaminating DNA. To prevent false positive PCR results, only DNA-free reagents should be used in PCR reactions with MTP Taq DNA polymerase.