Merck REDTaq DNA Polymerase

REDTaq® DNA Polymerase

Taq for routine PCR with inert dye, 10X buffer included

Quality Level

200

form

liquid

사용

sufficient for 1000 reactions
sufficient for 250 reactions
sufficient for 50 reactions

특징

dNTPs included: no
hotstart: no

농도

1 unit/μL

technique(s)

PCR: suitable

색상

red

input

purified DNA

application(s)

agriculture

배송 상태

wet ice

저장 온도

−20°C

REDTaq® DNA Polymerase is a unique blend of Sigma′s quality Taq DNA Polymerase combined with an inert red dye. This dye enables quick visual confirmation of enzyme addition and reaction mixing. An aliquot of the samples (5-10 μl) can then be loaded directly onto an agarose gel for electrophoresis following PCR. The red dye migrates slightly faster than bromophenol blue at about the same rate as a 125 base pair fragment in a 1% agarose gel. Since no additional loading buffers are added to the reaction following PCR, reamplification is possible.
The red dye has no effect on automated or manual sequencing, restriction digestions or other downstream applications. However, if removing the dye is desired, this can easily be accomplished using any standard purification method.