Merck ThermaStop-RT

ThermaStop™-RT

increases RT-PCR specificity, inhibits non-specific polymerase activity, prevents mis-priming before RT-PCR

hot start PCR, hotstart, PCR Additive, hot start DNA polymerase, Multiplex RT-PCR

assay

(Dual, HPLC)

form

lyophilized powder

포장

pkg of One unit of ThermaStop™-RT is defined as the amount required for optimal performance in a one-step RT-PCR containing 50 units of reverse transcriptase (RT) and one unit of hot-start Taq Polymerase in a volume of 20 ?L and a temperature of 50 degrees C.

척도

250 and 1250 (units)

technique(s)

RT-PCR: suitable

색상

opaque

배송 상태

ambient

저장 온도

2-8°C

ThermaStop™-RT is a proprietary additive for One-Step, or Two-Step Reverse Transcriptase-PCR assays containing gene-specific primers. ThermaStop™-RT interacts with the reverse transcriptase at low temperatures to reduce priming errors. ThermaStop™-RT improves detection sensitivity and specific product yield by eliminating priming errors at low temperature. ThermaStop-RT acts as a Hot-start which binds and inhibits DNA polymerase activity. It also renatures the DNA polymerase upon cooling and activates its functions at lower temperatures or post-amplification.