Merck Titan One Tube RT-PCR System

Titan One Tube RT-PCR System

sufficient for 100 reactions, kit of 1 (4 components), suitable for RT-PCR, hotstart: no

RT-PCR

Quality Level

100

사용

sufficient for 100 reactions

특징

dNTPs included: no
hotstart: no

포장

kit of 1 (4 components)

manufacturer/tradename

Roche

파라미터

42-60 °C optimum reaction temp.

technique(s)

RT-PCR: suitable

input

purified RNA

배송 상태

dry ice

저장 온도

−20°C

The Titan™ One Tube RT-PCR System uses Reverse Transcriptase (RT) Avian Myeloblastosis Virus (AMV) for first strand cDNA synthesis and the Expand™ High Fidelity enzyme blend consisting of Taq DNA Polymerase and a polymerase with a proofreading activity for amplification of cDNA by polymerase chain reaction (PCR). The proofreading activity, leads to an increased PCR fidelity. The Titan One Tube RT-PCR System:

• Reduces the error rate in PCR due to the proofreading capability. A threefold higher fidelity is obtained in comparision to Taq DNA polymerase.
• Delivers increased sensitivity due to the high efficiency of all three enzymes.
• Is faster than conventional two-step RT-PCR.