Merck GC-RICH PCR System

GC-RICH PCR System

sufficient for ≤50 reactions, pkg of 100 U, optimum reaction temp. 72 °C

GC-rich | PCR

Quality Level

100

사용

sufficient for ≤50 reactions

포장

pkg of 100 U

manufacturer/tradename

Roche

파라미터

72 °C optimum reaction temp.

배송 상태

dry ice

저장 온도

−20°C

The GC-RICH PCR System is composed of an enzyme blend of thermostable Taq DNA Polymerase and Tgo DNA Polymerase, a thermostable enzyme with a proofreading (3′-5′ exonuclease) activity. This polymerase mixture by itself outperforms Taq DNA Polymerase in respect to yields, fidelity and specificity beside the possibility to amplify fragments up to 5 kb in length. The GC-RICH PCR Reaction buffer in combination with the separately included GC-RICH resolution solution allows to amplify difficult templates like GC-rich targets very efficiently. TA cloning is recommended. The enzyme blend produces more blunt-ended fragments than Taq DNA Polymerase. The majority of products have single A overhangs. Use 2 U for a standard 50 μl PCR.

Contents

• GC-RICH Enzyme Mix , in storage buffer
• GC-RICH Reaction Buffer, 5x concentrated, with 7.5 mM MgCl2 and DMSO
• GC-RICH Resolution Solution, 5 M
• MgCl2 Stock Solution, 25 mM
• Water, PCR Grade