Merck MgCl2 Stock Solution

MgCl2 Stock Solution

pH 8.25-8.45 (25 °C), suitable for PCR, solution, pkg of 3 × 1 mL

magnesium chloride solution

form

solution

Quality Level

100

포장

pkg of 3 × 1 mL

manufacturer/tradename

Roche

농도

25 mM

technique(s)

PCR: suitable

pH

8.25-8.45 (25 °C)

배송 상태

dry ice

저장 온도

−20°C

The MgCl2 Stock Solution is used for the individual adjustment of the Mg2+-concentration in the PCR reaction and can be used in combination with the PCR buffer without MgCl2. In most applications a concentration of 1.5mM MgCl2 will yield satisfactory results with a dNTP concentration of 200μM each. In many cases, however, it is required to titrate the optimal Mg2+ concentration to increase the specificity and the yield of the PCR reaction.