Merck Lenti CRISPR Non-Target gRNA Control Transduction Particles, High Titer (LV02 vector)

Lenti CRISPR Non-Target gRNA Control Transduction Particles, High Titer (LV02 vector)

form

liquid

Quality Level

200

포장

vial of 8 x 25 μL

농도

(5x108 TU/ml (via p24 titering assay))

application(s)

CRISPR

배송 상태

dry ice

저장 온도

−70°C

The Lenti CRISPR Non-Target gRNA Control Transduction Particles (LV02 vector) include a gRNA sequence that does not target known human, mouse and rat genes. It is useful as a negative control in experiments using CRISPR lentiviral clones. The lenti CRISPR non-target control uses a dual-component system consisting of U6-driven guide RNA (non-target) and Ef1a-driven Puromycin resistance cassette. This control requires Cas9 be delivered by a separate vector.

Lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging and envelope plasmids.