Merck Lenti CRISPR Non-Target gRNA Control Transduction Particles, High Titer (LV02 vector)
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카탈로그 번호 | CAS 번호 | 설명 | 상태 | 단위 | 판매가 | 할인가 | 가격(VAT포함) | 수량 / 장바구니 / 찜 |
CRISPR15H-1EA | - | Merck CRISPR15H-1EA Lenti CRISPR Non-Target gRNA Control Transduction Particles, High Titer (LV02 vector), 1 EA pk | 재고문의 | pk | 1,223,040원 | - | 1,345,344원 |
다른 상품 둘러보기
Lenti CRISPR Non-Target gRNA Control Transduction Particles, High Titer (LV02 vector)
form
liquid
Quality Level
포장
vial of 8 x 25 μL
농도
(5x108 TU/ml (via p24 titering assay))
application(s)
CRISPR
배송 상태
dry ice
저장 온도
−70°C
The Lenti CRISPR Non-Target gRNA Control Transduction Particles (LV02 vector) include a gRNA sequence that does not target known human, mouse and rat genes. It is useful as a negative control in experiments using CRISPR lentiviral clones. The lenti CRISPR non-target control uses a dual-component system consisting of U6-driven guide RNA (non-target) and Ef1a-driven Puromycin resistance cassette. This control requires Cas9 be delivered by a separate vector.
Lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging and envelope plasmids.
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