Merck Lenti CRISPR Universal Non-Target Control#1 Plasmid DNA (LV04 vector)

Lenti CRISPR Universal Non-Target Control#1 Plasmid DNA (LV04 vector)

form

liquid

Quality Level

200

포장

vial of 50 μL

농도

20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)

application(s)

CRISPR

배송 상태

dry ice

저장 온도

−20°C

The Lenti CRISPR Non-Target Control#1 (LV04 vector) is a lentiviral plasmid vector, which includes a gRNA sequence that does not target known human, mouse and rat genes. The DNA format of this control construct permits direct transfection in knockout experiments or use in the viral packaging process (for lentiviral-based knockdown experiments). This vector is useful as a negative control in experiments using CRISPR lentiviral clones. The lenti CRISPR non-target control uses a dual-component system consisting of U6-driven guide RNA (non-targeting) and hPGK-driven Puromycin resistance cassette and Blue Fluorescence Protein for fluorophorescence. This control requires Cas9 be delivered by a separate vector.