Merck Human Kinase Lentiviral CRISPR Pool

Human Kinase Lentiviral CRISPR Pool

Quality Level

200

포장

pkg of 8x25 μL (vials)

농도

5x108 VP/ml (via p24 assay)

application(s)

CRISPR

배송 상태

dry ice

저장 온도

−70°C

Sigma CRISPR Human Kinase Pooled Lentiviral Library
Sigma-Aldrich has combined its long-standing expertise in pooled lentiviral screening with its CRISPR genome editing technology to create a powerful new discovery tool: the Sigma CRISPR human kinase pooled lentiviral library. This pooled library targets the human kinome, a collection of approximately 700 genes commonly associated with development and disease, with an average of nearly 9 unique knockout CRISPR clones per kinase gene. The lentiviral format allows for stable integration, selection, and enrichment of transduced cells, allowing researchers to functionally screen hundreds of kinase genes for a fraction of the time and cost of screening arrayed clones.
The Sigma CRISPR human kinase pooled lentiviral library uses a single lentiviral vector (pLV-U6g-EPCG) to deliver Cas9, gRNA, a puromycin selection marker, and GFP to target cells. The vector features a robust truncated human elongation factor−1 promoter alpha (tEF1a) driving expression of the Cas9 protein and a human U6 promoter ensuring the efficient transcription of gRNA in most mammalian cell types. The 2A peptides (P2A and T2A) flanking Cas9 allow stoichiometric expression of the puromycin resistance gene, Cas9, and GFP as individual proteins from a single transcript (see vector map below). Whether you are interested in small molecule screening or in defining developmental signaling pathways, Sigma′s CRISPR human kinase pooled lentiviral library offers researchers a high throughput solution to uncovering novel kinase targets that only a knockout screen can reveal.