Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

mmu-miR-453

형식: Tough Decoy (TuD)

제품 정보

제품 설명

Individual lenti microRNA inhibitors are designed using a proprietary algorithm based on the work of Haraguchi, T. et al. in collaboration with Dr. Hideo Iba (University of Tokyo).
This algorithm utilizes the Tough Decoy (TuD) design.

miRNA are known to regulate gene expression through translational repression, mRNA cleavage, and deadenylation.
The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector. Co-transfection of this vector into the appropriate cell line with compatible packaging plasmids produces viral particles suitable for mammalian cell transduction.

Additionally, the Woodchuck Hepatitis Post-Transcriptional Regulatory Element 2 (WPRE) is included to enhance transgene expression.
The lentiviral vector also carries a puromycin resistance gene for selection of transduced cells.

주요 특징

• 강력한 miRNA 억제 효과 제공
• 다양한 세포 유형에 효율적 전달 가능
• 반복적인 트랜스펙션 없이 장기적 억제 가능
• 퓨로마이신 내성 유전자를 통해 선택적 세포 배양 가능