Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

mmu-miR-301b-3p

Type: Tough Decoy (TuD)

제품 정보

제품 설명

Individual lenti microRNA inhibitors are designed using a proprietary algorithm based on the work of Haraguchi, T. et al. in collaboration with Dr. Hideo Iba (University of Tokyo).
This algorithm utilizes the Tough Decoy (TuD) design to achieve potent inhibition of specific miRNAs.

miRNAs regulate gene expression through mechanisms such as translational repression, mRNA cleavage, and deadenylation.
The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector.
Co-transfection of this vector with compatible packaging plasmids produces viral particles suitable for transduction into mammalian cells.

Additionally, the Woodchuck Hepatitis Post-Transcriptional Regulatory Element2 (WPRE) enhances transgene expression in lentiviral systems.
The vector also carries a puromycin resistance gene for selection.

주요 특징

• 강력한 miRNA 억제 가능
• 렌티바이러스 전달 형식으로 다양한 세포에 효율적 도입
• 반복적 형질감염 없이 장기적인 억제 유지
• Puromycin 선택 마커 포함
• −70°C에서 안정적 보관 및 건빙 상태 배송