Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

mmu-miR-468-5p

Tough Decoy (TuD)

제품 개요

Individual lenti microRNA inhibitors are designed using a proprietary algorithm based on the work of Haraguchi, T. et al., in collaboration with Dr. Hideo Iba (University of Tokyo).
This algorithm utilizes the Tough Decoy (TuD) design.

miRNA are known to regulate gene expression through:

• Translational repression
• mRNA cleavage
• Deadenylation

The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector.
Co-transfection of this vector into an appropriate cell line with compatible packaging plasmids produces viral particles that can transduce mammalian cells.

Additionally, the Woodchuck Hepatitis Post-Transcriptional Regulatory Element 2 (WPRE) is included to enhance transgene expression.
This vector also carries a puromycin resistance gene for cell selection.

주요 특징

• Potent inhibition of the desired miRNA
• Efficient lentiviral delivery into various cell types
• Enables long-term inhibition without repeat transfection

제품 사양