Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

대상 microRNA

• mmu-miR-3104-5p

디자인

• Tough Decoy (TuD) 방식 적용

제품 개요

Individual lenti microRNA inhibitors are designed using a proprietary algorithm based on the work of Haraguchi, T. et al. in collaboration with Dr. Hideo Iba (University of Tokyo).
This algorithm utilizes the Tough Decoy (TuD) design. miRNA are known to regulate gene expression through translational repression, mRNA cleavage, and deadenylation.

The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector.
Co-transfection with compatible packaging plasmids produces viral particles that can transduce mammalian cells.
The Woodchuck Hepatitis Post-Transcriptional Regulatory Element (WPRE) is included for enhanced transgene expression.
This vector also carries a puromycin resistance gene for selection of transduced cells.

주요 특징

• 강력한 miRNA 억제 효과
• 렌티바이러스 전달 포맷으로 다양한 세포 유형에 적용 가능
• 반복적인 트랜스펙션 없이 장기 억제 가능

제품 스펙