Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

mmu-miR-10a-3p

Tough Decoy (TuD) Design

품질 등급

200

제품 라인

MISSION®

형태

Liquid

농도

≥1×10⁶ VP/ml (via p24 assay)

성숙 서열

CAAAUUCGUAUCUAGGGGAAUA

Sanger 성숙/소수 수납 번호

MIMAT0004659

microRNA 수납 번호

MI0000685

배송 상태

Dry ice

저장 온도

−70°C

제품 설명

Individual lenti microRNA inhibitors are designed using a proprietary algorithm, based on the work of Haraguchi, T. et al., in collaboration with Dr. Hideo Iba (University of Tokyo).
This algorithm utilizes the Tough Decoy (TuD) design, enabling potent inhibition of target miRNAs.

miRNAs regulate gene expression through translational repression, mRNA cleavage, and deadenylation.
The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector. Co-transfection with compatible packaging plasmids produces viral particles suitable for mammalian cell transduction.

The vector includes:

• WPRE (Woodchuck Hepatitis Post-Transcriptional Regulatory Element 2) for enhanced transgene expression
• Puromycin resistance gene for selection of transduced cells

주요 특징

• 강력한 miRNA 억제 효과 제공
• 다양한 세포 유형에 효율적으로 전달 가능한 렌티바이러스 포맷
• 반복적인 트랜스펙션 없이 장기적인 억제 가능

제품 사양 요약